Ulcers on the glans penis should be checked promptly, because the symptoms of ulcers are similar to those of other skin diseases. Because the site of the disease is quite special, if it is not treated in time, if it is caused by a virus infection, the virus may enter the body through the urethra. 1. Examination and testing: For candidal glansitis (balanitis), Candida can be found by microscopic examination or culture of samples taken from the diseased glans and foreskin. For trichomonal glansitis, Trichomonas can be found in the secretions. 2. Direct microscopic examination: scrape the scales from the skin lesions on the glans penis, coronal sulcus or foreskin as the specimens to be tested. Prepare the specimens with 10% potassium hydroxide or saline, and groups of oval spores and pseudohyphae can be seen under the microscope. If more pseudohyphae are found, it means that Candida is in the pathogenic stage. 3. Staining examination: Gram staining, Congo red staining or PAS staining can also be used for microscopic examination, and the positive rate is higher than that of direct microscopic examination. Gram staining, spores and pseudohyphae are stained blue; Congo red and PAS staining, spores and pseudohyphae are stained red. 4. Separation culture: Patients with negative smear tests can undergo Candida culture. Inoculate the specimen on Sabouraud culture medium under sterile conditions. Cut the test tube culture medium slightly at an angle during inoculation. Inoculate 2-3 places in each tube, and inoculate 2 tubes for each specimen. Place the culture medium in a 37°C incubator and incubate for 24-48 hours. A large number of milky white colonies can be seen growing. Use an inoculation needle to pick up a small amount of colonies for smear. Direct microscopy or microscopy after staining can reveal a large number of spores, which can be preliminarily diagnosed as Candida infection. |
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